細胞名稱

人膀胱移行細胞癌UM-UC-3

英文名稱

UM-UC-3

形態(tài)特性

上皮細胞樣

生長特性

貼壁生長

種屬

人

組織

膀胱

培養(yǎng)體系

MEM＋10% FBS＋1% P/S     氣相：空氣，95%；CO2，5%。  溫度：37℃

STR

Amelogenin: X CSF1PO: 10,11 D13S317: 8 D16S539: 8,9 D5S818: 12 D7S820: 8,9 THO1: 6,9 TPOX: 10 vWA: 17

簡介

Derived from a urinary bladder transitional cell carcinoma from a human male. These cells are tumourigenic in nude mice when 10E7 cells are inoculated subcutaneously. Hypertriploid. The modal chromosome number was 80, occurring in 42% of cells. Cells with 78 chromosomes also occurred at a high frequency. The rate of cells with higher ploidies was 2.5%. There were 30 or more marker chromosomes in each cell. They included der(1)t (1;?) (p32;?), ?t(1p5p), i(3q), t(7q14q), ?t(2p3p) and others. The X and N3 had single copy per cell, and others were generally two or three copies per cell.

致瘤性

Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.

傳代方法

建議第一次1:2傳代

凍存條件

90 FBS+10% DMSO,  推薦無血清凍存液 (CX001)

用途

僅限于科學(xué)研究，不可作為動物或人類疾病的治療產(chǎn)品使用。